| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 158 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ROCK1 Rabbit Polyclonal Antibody detects endogenous levels of ROCK1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ROCK1 is a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. ROCK1 interacts with several key proteins, including myosin light chain (MLC) to regulate contractility, LIM kinase to influence actin filament dynamics, and the small GTPase RhoA, which activates ROCK1 and modulates various downstream signaling pathways. These interactions make ROCK1 pivotal in maintaining cell shape, enabling signal transduction pathways that affect cell proliferation, apoptosis, and migration, and playing significant roles in physiological and pathological processes such as cancer metastasis, cardiovascular diseases, and neurological disorders. ROCK1 monoclonal antibody (B-1) facilitates the detailed study of these interactions by providing consistent and precise detection of ROCK1 in diverse experimental settings. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7 cells, using ROCK1 Rabbit Polyclonal Antibody (EAB11282) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human breast cancer tissue, using ROCK1 Rabbit Polyclonal Antibody (EAB11282) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using ROCK1 Rabbit Polyclonal Antibody (EAB11282)(green) at 1:200 dilution.
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