| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 36 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Annexin A5 Mouse Monoclonal antibody detects endogenous levels of Annexin A5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Annexin A5 also known as Annexin V, ANXA5 is a member of the annexin family of calcium-binding proteins, characterized by a conserved core domain that forms a propeller-like structure essential for binding phospholipids in a calcium-dependent manner, and a distinct N-terminal domain that provides binding specificity and facilitates interactions with other cellular proteins. The structural integrity of Annexin V is vital for regulating membrane dynamics, apoptotic cell recognition by binding to phosphatidylserine, and involvement in processes such as ion flux, endocytosis, exocytosis, and cellular adhesion. Annexin V′s ability to form ion channels and regulate protein kinase C (PKC) activity underscores its importance in cellular signaling pathways and membrane organization. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, A431, HepG2, NIH/3T3 cells, using Annexin A5 Mouse Monoclonal antibody (EAB22543) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunofluorescence analysis of Hela cell, using Annexin A5 Mouse Monoclonal antibody (EAB22543)(green) at 1:200 dilution.
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