| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 211 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MYLK Mouse Monoclonal Antibody detects endogenous levels of MYLK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MYLK also known as MLCK is a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. MYLK protein plays a crucial role in muscle contraction by phosphorylating myosin regulatory light chains, which facilitates interaction between myosin and actin filaments, leading to contractile activity. This function is particularly important in smooth muscle tissues, where MYLK isoforms contribute to regulation of vascular tone and gastrointestinal motility. MYLK gene encodes several isoforms, including those specific to smooth muscle and non-muscle tissues, as well as telokin, a small isoform that stabilizes unphosphorylated myosin filaments. Multiple transcript variants for MYLK gene highlight diverse regulatory roles across different tissues. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, C2C12, PC3 cells, using MYLK Mouse Monoclonal Antibody (EAB22646) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, using MYLK Mouse Monoclonal Antibody (EAB22646) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using MYLK Mouse Monoclonal Antibody (EAB22646)(green) at 1:200 dilution.
 Flow cytometric analysis of Hela cell, using MYLK Mouse Monoclonal Antibody (EAB22646)(green) and negative control (red).
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