| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 103 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:200-1000 IF/ICC=1:100-500 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | α-Actinin Mouse Monoclonal Antibody detects endogenous levels of α-Actinin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
α-actinin also known as Actinin alpha 1, ACTN1 is a crucial cytoskeletal protein that plays a significant role in maintaining cellular structural integrity by cross-linking actin filaments, thereby forming a stable network that supports cellular shape and function. α-actinin is predominantly located in the cytoskeleton of muscle and non-muscle cells, including skeletal, cardiac, and smooth muscle cells, where α-actinin is essential for muscle contraction and cellular signaling. Proper localization of α-actinin is vital for muscle function and cellular organization, as α-actinin anchors actin filaments to various intracellular structures, facilitating the transmission of mechanical forces and signals within the cell. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HEK293, Raw264.7 cells, using α-Actinin Mouse Monoclonal Antibody (EAB22699) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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