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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 164 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-FANCD2 (Ser222) Rabbit Polyclonal Antibody detects endogenous levels of FANCD2 protein only when phosphorylated at Ser222. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FANCD2 is part of the Fanconi anemia (FA) pathway, a critical cellular mechanism that maintains genomic integrity. FANCD2 plays a crucial role in the DNA damage response, particularly in the repair of interstrand cross-links, which are a type of DNA damage that can lead to genomic instability and cancer if not properly repaired. When DNA is damaged, FANCD2 undergoes monoubiquitination, which is essential for activation and subsequent localization to sites of DNA damage, where FANCD2 interacts with other FA proteins and the breast cancer susceptibility protein BRCA1. This interaction is vital for the recruitment of repair factors to the damaged DNA, thereby facilitating effective repair processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on HT29 cells treated with Calyculin A, using Phospho-FANCD2 (Ser222) Rabbit Polyclonal Antibody (EAB11251) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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