| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 312 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | APC Rabbit Monoclonal Antibody detects endogenous levels of APC protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
APC is a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. APC plays a crucial role in regulating cell adhesion and maintaining the integrity of epithelial tissues, which is vital for preventing tumorigenesis. Mutations in APC gene are commonly associated with familial adenomatous polyposis and Gardner′s syndrome, conditions characterized by the development of numerous adenomatous polyps that can lead to colon cancer. APC protein, consisting of 2,843 amino acids, is ubiquitously expressed and interacts with key structural components of intracellular junctions, including β-catenin and γ-catenin, which are essential for cellular adhesion. APC competes with E-cadherin for binding to specific regions of β- and γ-catenin, and interactions between cytoskeleton and APC-E-cadherin-β/γ-catenin complex are mediated by α-catenin, highlighting APC′s importance in cellular signaling pathways that regulate cell growth and differentiation. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, C2C12 cells, using APC Rabbit Monoclonal Antibody (EAB21200) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 312 kDa; Observed band size: 160 kDa |
