| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 86 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TPX2 Rabbit Monoclonal Antibody detects endogenous levels of TPX2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TPX2(Targeting protein for Xklp2) is a crucial microtubule-associated protein that plays a significant role in the assembly of microtubules during cell division, particularly in the M phase of the cell cycle. TPX2 is activated by Ran-GTP, which facilitates chromatin-induced microtubule assembly, ensuring proper spindle formation and function. The structural integrity of TPX2 is vital as TPX2 interacts with Aurora-A kinase at the spindle apparatus, where TPX2 may be regulated through phosphorylation, further emphasizing TPX2′s importance in mitotic spindle assembly. Additionally, TPX2 activates Eg2, a kinase that is essential for microtubule dynamics, by enhancing Eg2′s phosphorylation and activity in a microtubule-dependent manner. Notably, TPX2′s function can be inhibited by binding to importin α, a nuclear import factor, which highlights the regulatory mechanisms that control TPX2′s activity. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Raw264.7, Raji cells, using TPX2 Rabbit Monoclonal Antibody (EAB21180) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 86 kDa; Observed band size: 100 kDa |
