| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | IHC-P, IF/ICC, FC, ELISA | 75 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MELK Mouse Monoclonal antibody detects endogenous levels of MELK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MELK(Maternal embryonic leucine zipper kinase) also known as KIAA0175, HPK38 is a member of the Snf1/AMPK family of kinases, characterized by a kinase catalytic domain and a distinctive leucine zipper motif that consists of periodic repetition of leucine residues at every seventh position within the N-terminal catalytic domain. This structural motif is significant as leucine zippers are often found in various DNA-binding proteins, suggesting involvement in crucial protein-DNA and protein-protein interactions. MELK plays a key role in signal transduction events during egg and early embryo stages of development. Mouse and human MELK proteins share 95% sequence identity in the kinase domain, demonstrating high conservation. |
| Product Image Gallery | |
 Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue, using MELK Mouse Monoclonal antibody (EAB22549) at 1:200 dilution.
 Immunofluorescence analysis of HepG2 cell, using MELK Mouse Monoclonal antibody (EAB22549)(green) at 1:200 dilution.
|
