| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 75 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MELK Rabbit Polyclonal Antibody detects endogenous levels of MELK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MELK(Maternal embryonic leucine zipper kinase) also known as KIAA0175, HPK38 is a member of the Snf1/AMPK family of kinases, characterized by a kinase catalytic domain and a distinctive leucine zipper motif that consists of periodic repetition of leucine residues at every seventh position within the N-terminal catalytic domain. This structural motif is significant as leucine zippers are often found in various DNA-binding proteins, suggesting involvement in crucial protein-DNA and protein-protein interactions. MELK plays a key role in signal transduction events during egg and early embryo stages of development. Mouse and human MELK proteins share 95% sequence identity in the kinase domain, demonstrating high conservation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurat, K562 cells, using MELK Rabbit Polyclonal Antibody (EAB11182) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using MELK Rabbit Polyclonal Antibody (EAB11182) at 1:200 dilution.
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