| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 49 | Human, Mouse, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Legumain Mouse Monoclonal Antibody detects endogenous levels of Legumain protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Legumain also known as LGMN, AEP, PRSC1 is a 433 amino acid protein that localizes to the lysosome and belongs to the peptidase C13 family. Expressed ubiquitously with particularly high expression in placenta, heart and kidney, Legumain functions as a cysteine protease that specifically catalyzes the hydrolysis of asparaginyl and aspartyl bonds. Additionally, Legumain is thought to be involved in the processing of bacterial proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Legumain exists as both a precursor and a fully mature, active enzyme that is produced in dendritic cells. Overexpression of Legumain may be associated with the formation of solid tumors, suggesting a role for Legumain in carcinogenesis. Multiple isoforms of Legumain exist due to alternative splicing events. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, MCF7 cells, using Legumain Mouse Monoclonal Antibody (EAB22693) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 49 kDa; Observed band size: 36 kDa |
