| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 53 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MeCP2 Mouse Monoclonal Antibody detects endogenous levels of MeCP2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MeCP2 plays a crucial role in regulating gene expression through binding to methylated DNA, which is essential for normal neurological function. Mutations in MeCP2 are linked to Rett syndrome, a severe neurodevelopmental disorder primarily affecting females, highlighting MeCP2′s importance in maintaining cognitive and motor functions. MeCP2 is predominantly located in the nucleus, where MeCP2 interacts with methylated DNA and various corepressor complexes, including mSin3A and histone deacetylases, to mediate transcriptional repression. This nuclear localization enables MeCP2 to effectively regulate gene expression in response to DNA methylation changes, which are critical for neuronal development and plasticity. MeCP2′s ability to bind tightly to methylated regions of the genome underscores MeCP2′s significance in epigenetic regulation and potential impact on various neurological disorders when dysregulated. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, MCF7 cells, using MeCP2 Mouse Monoclonal Antibody (EAB21191) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue, using MeCP2 Mouse Monoclonal Antibody (EAB21191) at 1:200 dilution.
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