| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 96 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:100-500 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Axin1 Mouse Monoclonal Antibody detects endogenous levels of Axin1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Axin1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. Axin1 plays a crucial role in the regulation of the Wnt signaling pathway, which is essential for various cellular processes, including cell proliferation, differentiation, and migration. Axin1 functions as a scaffold that facilitates the formation of a complex with β-catenin, adenomatous polyposis coli (APC), and glycogen synthase kinase 3 beta (GSK-3β), promoting the degradation of β-catenin. This degradation is vital for preventing aberrant Wnt signaling, which can lead to developmental disorders and cancer. Additionally, Axin1′s structural similarity to Conductin, which shares 45% identity, suggests that Axin may have overlapping functions in Wnt signaling regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, A431, HepG2, Jurkat cells, using Axin1 Mouse Monoclonal Antibody (EAB22679) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 96 kDa; Observed band size: 110 kDa |
