| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 14 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:50-300 IF/ICC=1:50-300 FC=1:20-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HINT1 Mouse Monoclonal Antibody detects endogenous levels of HINT1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HINT1 also known as PRKCNH1, PKCI1 is a 126 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one HIT (histidine triad) domain. Widely expressed and existing as a homodimer, HiINT1 functions to hydrolyze adenosine 5′-monophosphoramidate substrates, such as AMP-N-alanine methyl ester, AMP-morpholidate and AMP-alpha-acetyl lysine methyl ester, and may also inhibit PKC function and influence apoptosis. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji, Jurkat, HepG2, Mouse liver, Rat brain cells, using HINT1 Mouse Monoclonal Antibody (EAB22677) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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