| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 57 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HARS Rabbit Polyclonal Antibody detects endogenous levels of HARS protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HARS also known as HisRS, HARS1 is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. HARS plays a crucial role in protein synthesis by charging transfer RNAs (tRNAs) with histidine, which is essential for the incorporation of this amino acid into proteins. Proper functioning of HARS ensures proteins are synthesized correctly, which is particularly important in maintaining cellular functions and overall organismal health. Dysregulation of HARS can lead to the production of autoantibodies in systemic autoimmune diseases, where the immune system mistakenly targets its own proteins. HARS is recognized as an antigen in conditions such as rheumatic arthritis and polymyositis, which can be associated with interstitial lung disease, thrombocytopenia, and Raynaud′s phenomenon, a condition characterized by vasospastic attacks that constrict blood vessels. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205 cells, using HARS Rabbit Polyclonal Antibody (EAB11153) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using HARS Rabbit Polyclonal Antibody (EAB11153) at 1:200 dilution.
 Immunofluorescence analysis of HepG2 cell, using HARS Rabbit Polyclonal Antibody (EAB11153) at 1:200 dilution.
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