| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 53 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cytokeratin 14 Mouse Monoclonal Antibody detects endogenous levels of Cytokeratin 14 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cytokeratin 14 also known as Keratin 14, CK14, KRT14 is a member of the keratin family, the most diverse group of intermediate filaments. Cytokeratin 14 plays a crucial role in maintaining cellular integrity and resilience. Cytokeratin 14 is predominantly located in the basal layer of the epidermis, forming part of the intermediate filament network that provides mechanical support and stability to keratinocytes. Cytokeratin 14 expression helps protect against mechanical stress and contributes to the skin′s barrier function. Mutations in Cytokeratin 14 can lead to conditions such as epidermolysis bullosa simplex, a genetic disorder characterized by skin fragility and blistering. Cytokeratin 14 serves as a marker for epithelial differentiation and aids in diagnosing various skin malignancies, including Bowen′s disease, where expression patterns can indicate neoplastic changes. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431 cells, using Cytokeratin 14 Mouse Monoclonal Antibody (EAB22609) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using Cytokeratin 14 Mouse Monoclonal Antibody (EAB22609) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Human prostate tissue, using Cytokeratin 14 Mouse Monoclonal Antibody (EAB22609) at 1:200 dilution.
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