| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IF/ICC, ELISA | 88 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Golgin-97 Mouse Monoclonal Antibody detects endogenous levels of Golgin-97 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Golgin-97 also known as GOLGA1 is a crucial component of the trans-Golgi network, functioning as a peripheral membrane protein characterized by an extensive coiled-coil structure, comprising approximately 67% α-helical content, and a C-terminal GRIP domain. This unique structure allows golgin 97 to effectively localize to the cytoplasmic face of the Golgi apparatus, where golgin 97 plays a vital role as a tethering molecule that associates with tubulovesicular carriers during trafficking from the trans-Golgi network to recycling endosomes and early endosomes. The interaction of golgin 97 with the Golgi membrane is facilitated by the G protein family member, Arl1, highlighting golgin 97′s importance in maintaining cellular organization and transport. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, A549 cells, using Golgin-97 Mouse Monoclonal Antibody (EAB22669) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 88 kDa; Observed band size: 97 kDa |
