| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | WB, IF/ICC, ELISA | 23 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ASF1A Rabbit Polyclonal Antibody detects endogenous levels of ASF1A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ASF1A also known as anti-silencing function 1A is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. plays a crucial role as a transcription factor that interacts with core histones H3 and H4 H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. , facilitating gene expression regulation. This interaction is vital for proper nucleosome assembly, which is essential for DNA packaging and gene regulation. ASF1A becomes highly phosphorylated during S-phase of the cell cycle, a modification critical for chromatin dynamics and gene activation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using ASF1A Rabbit Polyclonal Antibody (EAB11116) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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