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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 52 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PDCD4 (Ser457) Rabbit Polyclonal Antibody detects endogenous levels of PDCD4 protein only when phosphorylated at Ser457. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PDCD4(Programmed cell death 4) is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. PDCD4 plays a crucial role in regulating apoptosis and functions as a tumor suppressor gene, particularly in lung cancer. PDCD4 inhibits the transformation of mouse keratinocytes induced by tumor promoters, highlighting its importance in cancer biology. PDCD4 is primarily located in the nucleus, where PDCD4 interacts with key regulatory proteins involved in gene expression and translation. Notably, PDCD4 directly binds to RNA helicase eIF4A, inhibiting protein synthesis by disrupting the formation of the cap-dependent translation initiation complex. This interaction underscores PDCD4′s significance in controlling cellular growth and proliferation, as PDCD4 also suppresses c-Jun activity, a critical transcription factor involved in oncogenic signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293 cells treated with Serum, using Phospho-PDCD4 (Ser457) Rabbit Polyclonal Antibody (EAB13731) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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