| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 81 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cyclin T1 Rabbit Polyclonal Antibody detects endogenous levels of Cyclin T1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cyclin T1 also known as CCNT1 is a member of the highly conserved cyclin C subfamily. Cyclin T1 plays a crucial role in regulating transcription elongation by forming a complex with cyclin-dependent kinase 9 (Cdk9), which is essential for phosphorylating the C-terminal domain of RNA polymerase II. This phosphorylation enables RNA polymerase II to transition from transcription initiation to elongation, facilitating mRNA production. Cyclin T1 also interacts with HIV-1 Tat protein, enhancing its binding affinity to the viral TAR RNA structure, which is critical for recruiting the cyclin T1/Cdk9 complex to RNA polymerase II. The specificity of cyclin T1 in this interaction highlights its importance in both cellular transcription processes and viral pathogenesis, making cyclin T1 a significant target for research in gene expression and HIV biology.
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| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat cells, using Cyclin T1 Rabbit Polyclonal Antibody (EAB11099) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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