| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 34 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:200-1000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CYB5R3 Mouse Monoclonal Antibody detects endogenous levels of CYB5R3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CYB5R3(Cytochrome b5 reductase 3) also known as DIA1 consists of 301 amino acids and is encoded by the human gene CYB5R3. CYB5R3 plays a crucial role in the desaturation and elongation of fatty acids, cholesterol biosynthesis, and drug metabolism, making CYB5R3 essential for maintaining cellular lipid homeostasis and detoxifying harmful substances. Additionally, in erythrocytes, CYB5R3 is vital for the reduction of methemoglobin, which is important for ensuring efficient oxygen transport in the blood. Notably, a serine residue at position 117 of CYB5R3 is predominantly found in individuals of African descent, highlighting the potential for genetic variation in enzyme function and its implications for health. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, Jurkat cells, using CYB5R3 Mouse Monoclonal Antibody (EAB22651) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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