| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 46 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HSP47/SERPINH1 Mouse Monoclonal Antibody detects endogenous levels of HSP47/SERPINH1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HSP47 also known as CBP1, SERPINH1 is a crucial molecular chaperone that plays a significant role in the biosynthesis of collagen type I. HSP47 is predominantly located in the endoplasmic reticulum of cells that produce procollagen, where HSP47 assists in the proper folding and assembly of collagen molecules, ensuring their structural integrity and functionality. HSP47′s location is essential for the secretion of procollagen from these cells, thereby influencing the extracellular matrix formation and tissue repair processes. Additionally, HSP47 acts as a stress response molecule, sequestering abnormal procollagen following cellular insult, which highlights HSP47′s protective role in maintaining collagen homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, NIH/3T3, 4T1 cells, using HSP47/SERPINH1 Mouse Monoclonal Antibody (EAB22647) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
