| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 56 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NFIC Rabbit Polyclonal Antibody detects endogenous levels of NFIC protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NFIC also known as NF1C is a member of the NF1 (nuclear factor 1) family of transcription factors. NFIC plays a crucial role in regulating gene expression by stimulating DNA replication and activating transcription. This 508 amino acid protein is localized in the nucleus and features a CTF/NF-I DNA-binding domain, allowing formation of homodimers that bind to specific DNA sequences, such as the palindromic sequence 5′-TTGGCNNNNNGCCAA-3′. Such binding influences transcriptional regulation of both cellular and viral promoters, thereby impacting various biological processes, including TGFβ signaling and extracellular matrix deposition. NFIC is expressed in multiple tissues, including brain, liver, spleen, and heart, and exists in five alternatively spliced isoforms, highlighting diverse functional roles in cellular physiology and pathology. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A549 cells, using NFIC Rabbit Polyclonal Antibody (EAB11064) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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