| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, FC, ELISA | 47 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | E2F1 Mouse Monoclonal antibody detects endogenous levels of E2F1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
E2F1 also known as RBP3 is a member of the E2F family of transcription factors. E2F1 plays a critical role in both normal cell cycle control and in the dysregulation of cell proliferation seen in various malignancies. E2F1 forms functional heterodimers with DP-1, and this complex drives the expression of genes that promote cell cycle progression and DNA replication. E2F1 is particularly important in regulating the transition from the G1 phase to the S phase, and E2F1 activity is tightly controlled by interactions with the retinoblastoma protein (Rb). Rb binding inhibits E2F-1 transcriptional activity, preventing the expression of cell cycle genes until appropriate signals trigger Rb inactivation, a process often disrupted in cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, SKNSH, NIH/3T3 cells, using E2F1 Mouse Monoclonal antibody (EAB22515) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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