| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 162 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:2000-10000 IHC-P=1:200-1000 IF/ICC=1:200-1000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | EEA1 Mouse Monoclonal antibody detects endogenous levels of EEA1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
EEA1(Early endosome antigen 1) is a marker of early endosomes that plays a crucial role in the early endosomal pathway, which is essential for sorting and transport of endocytosed proteins. EEA1 is primarily located in the cytoplasm, where EEA1 associates with early endosomes, and proper localization is vital for regulation of membrane trafficking processes. EEA1 contains a calmodulin-binding IQ motif and a cysteine-rich finger motif, both necessary for specific localization to early endosomes. EEA1 interacts with transferrin receptor and Rab5, highlighting EEA1′s importance in the endocytic pathway. EEA1′s structure and interactions with other proteins are critical for maintaining cellular homeostasis and facilitating recycling of membrane proteins, making EEA1 a significant target for studies related to membrane dynamics and trafficking disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HKE293, A431 cells, using EEA1 Mouse Monoclonal antibody (EAB22519) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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