| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 110 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:200-1000 IF/ICC=1:100-500 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | APN/CD13 Mouse Monoclonal antibody detects endogenous levels of APN/CD13 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD13 also known as APN, ANPEP is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. CD13 functions as a zinc-binding metalloprotease, playing a crucial role in the digestion of peptides and the inactivation of regulatory peptides like enkephalins, which are important for pain modulation and stress response. CD13 is significant in cancer biology, as CD13 may enhance the invasive capacity and metastatic behavior of cancer cells, making CD13 a potential target for therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on U937 cells, using APN/CD13 Mouse Monoclonal antibody (EAB22581) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 110 kDa; Observed band size: 150 kDa |
