| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 118 | Human, Mouse, Rat, Chicken |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NLRP3 Mouse Monoclonal Antibody detects endogenous levels of NLRP3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NLRP3 also known as Cryopyrin, NALP3 is a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. NLRP3 plays a vital role in the innate immune response by detecting pathogenic microorganisms and cellular stress. Structurally, NLRP3 contains a central nucleotide-binding and oligomerization domain (NACHT), multiple leucine-rich repeats (LRRs) at the C-terminus, and an N-terminal pyrin domain (PYD), which are essential for assembling and activating the inflammasome. Post-translational modifications, such as phosphorylation and ubiquitination, regulate NLRP3′s activity and stability, thereby controlling the activation of caspase-1 and the subsequent processing and release of pro-inflammatory cytokines like IL-1β and IL-18. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1, Raw264.7 cells, using NLRP3 Mouse Monoclonal Antibody (EAB22645) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 118 kDa; Observed band size: 80-120 kDa |
