| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 118 | Human, Mouse, Rat, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:200-1000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GLI1 Mouse Monoclonal Antibody detects endogenous levels of GLI1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GLI1 is a member of the Kruppel family of zinc finger proteins. GLI1 plays a pivotal role in the Hedgehog signaling pathway, which is critical for embryonic development, tissue patterning, and the maintenance of stem cell populations. Located primarily in the nucleus, GLI1 functions as a transcriptional activator by binding to specific DNA sequences and regulating the expression of target genes involved in cell proliferation, differentiation, and survival. Nuclear localization of GLI1 is essential for modulating gene expression, and dysregulation is implicated in various cancers, including gliomas, highlighting GLI1′s importance in both normal physiology and oncogenic processes. GLI1 interacts with other proteins such as SUFU and various co-activators to finely tune transcriptional activity, ensuring precise control of cellular responses to developmental and environmental cues. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, HEK293, A549 cells, using GLI1 Mouse Monoclonal Antibody (EAB21207) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 118 kDa; Observed band size: 150 kDa |
