| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 70 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HSP70 Rabbit Polyclonal Antibody detects endogenous levels of HSP70 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HSP70 also known as HSPA1A is a 70kDa heat shock protein which is a member of the heat shock protein 70 family. HSP70 plays a vital role in cellular stress responses, particularly in protecting cells from damage caused by heat and other stressors. HSP70 functions primarily as a molecular chaperone, facilitating the proper folding of nascent polypeptide chains and preventing the aggregation of misfolded proteins, which is essential for maintaining cellular homeostasis. HSP70 is predominantly located in the cytosol and nucleus of mammalian cells, where HSP70 assists in the translocation of proteins across cellular membranes and into organelles, thereby ensuring that proteins reach their correct destinations and maintain their functional conformations. HSP70′s ability to respond to stress by increasing expression levels is critical for cell survival, especially under conditions that induce protein misfolding. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, T47D, Rat lung cells, using HSP70 Rabbit Polyclonal Antibody (EAB11888) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human Breast cance tissue, using HSP70 Rabbit Polyclonal Antibody (EAB11888) at 1:100 dilution.
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