| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 26 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD8a Mouse Monoclonal Antibody detects endogenous levels of CD8a protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD8a also known as CD8α, Leu-2, T8, CD8alpha is a transmembrane glycoprotein expressed on the surface of cytotoxic T lymphocytes and some natural killer cells. CD8a plays a crucial role in adaptive immune response. Structurally, CD8a forms either homodimers (αα) or heterodimers with CD8-β (αβ), featuring an extracellular immunoglobulin-like domain that binds to the α3 domain of major histocompatibility complex (MHC) class I molecules presented by antigen-presenting cells (APCs). This structural configuration facilitates interaction between T cells and APCs, enhancing antigen recognition and signal transduction through T cell receptor (TCR). Any alterations in CD8a′s structure can affect immune surveillance and cytotoxic T cells′ ability to identify and eliminate infected or malignant cells. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse thymus cells, using CD8a Mouse Monoclonal Antibody (EAB21325) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using CD8a Mouse Monoclonal Antibody (EAB21325) at 1:200 dilution.
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