| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 47 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PTEN Mouse Monoclonal Antibody detects endogenous levels of PTEN protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PTEN also known as phosphatase and tensin homolog, MMAC1, TEP1 is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. PTEN plays a vital role in regulating cellular processes by dephosphorylating phosphatidylinositol (3,4,5)-trisphosphate, thereby antagonizing the PI3K/AKT signaling pathway, which is essential for controlling cell growth, proliferation, and survival. The proper function of PTEN is further regulated through various post-translational modifications, including phosphorylation and ubiquitination, which influence stability, localization, and enzymatic activity. Dysregulation or mutation of PTEN, often observed in numerous cancers such as glioblastomas, breast, kidney, and prostate cancers, underscores its importance in maintaining cellular homeostasis and preventing tumorigenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, NIH/3T3 cells, using PTEN Mouse Monoclonal Antibody (EAB21199) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 47 kDa; Observed band size: 54 kDa |
