| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 57 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | B7-H3/CD276 Rabbit Monoclonal Antibody detects endogenous levels of B7-H3/CD276 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
B7-H3 also known as B7RP-2, CD276 is a member of the B7 Immunoglobulin superfamily. B7-H3 plays a crucial role in the regulation of T cell activation and immune responses. B7-H3 is widely expressed in non-lymphoid tissues, where B7-H3 serves as a costimulatory molecule that enhances the proliferation of both CD4+ and CD8+ T cells. Notably, B7-H3 has been shown to stimulate Th1 and cytotoxic T cell responses, suggesting potential antitumor activity. B7-H3 interactions with receptors may be pivotal in modulating cell-mediated immune responses against cancer, positioning B7-H3 as a promising candidate for therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T, MCF7 cells, using B7-H3/CD276 Rabbit Monoclonal Antibody (EAB21198) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 57 kDa; Observed band size: 57, 90-110 kDa |
