Antibiotics - Puromycin dihydrochloride (CAS: 58-58-2)

Puromycin dihydrochloride (CAS: 58-58-2)

Catalog #：EBC51170

Biological Activity

Synonyms

CL13900 dihydrochloride; Stylomycin hydrochloride; 嘌呤霉素;

Chemical Name

(2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride

Application

Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

CAS Number

58-58-2

Purity

≥98.0%

Molecular Weight

544.43

Molecular Formula

C₂₂H₂₉N₇O₅•2HCl

SMILES

CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Target & IC50

Aminoglycoside

Shipping

Gel Pack

Storage

Store at -20°C

Preparing Stock Solutions

Molecular Structure

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	1.8368 ml	9.1839 ml	18.3678 ml
5 mM	0.3674 ml	1.8368 ml	3.6736 ml
10 mM	0.1837 ml	0.9184 ml	1.8368 ml
50 mM	0.0367 ml	0.1837 ml	0.3674 ml

Solubility
DMSO: 50 mg/mL (20 mM) Water: 50 mg/mL (20 mM)

Product Description

Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation. Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase.