| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 98 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | F4/80 Mouse Monoclonal antibody detects endogenous levels of F4/80 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at +4°C |
| UniProt ID | Q14246 Human Q61549 Mouse |
| Entrez Gene ID | 2015 Human 13733 Mouse |
| Product Description | |
F4/80 also known as EMR1, ADGRE1 is a member of the epidermal growth factor (EGF)-TM7 family of G-protein coupled receptors, characterized by extended extracellular regions containing multiple EGF-like domains. F4/80 protein is exclusively expressed on macrophage surfaces, serving as a critical marker for mature macrophage populations in various tissues, including Kupffer cells in the liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 expression on macrophages is vital for understanding immune responses, playing a role in identifying and characterizing these immune cells in both health and disease. F4/80/EMR1 undergoes significant post-translational modifications, including extensive N-linked glycosylation and some O-linked glycosylation, which may influence macrophage adhesion, migration, and signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on HL60 cells, using F4/80 Mouse Monoclonal antibody (EAB22201) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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