| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 82 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SirT1 Rabbit Polyclonal Antibody detects endogenous levels of SirT1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SIRT1 also known as SIR2, SIR2L1, SIR2alpha is a member of the sirtuin family. SIRT1 possesses a conserved catalytic core composed of a large Rossmann-fold domain and a smaller zinc-binding domain, interconnected by a flexible linker region that is essential for NAD⁺-dependent deacetylase activity. This structural configuration allows SIRT1 to interact with a variety of substrates, including histones and transcription factors, thereby regulating key cellular processes such as gene expression, DNA repair, metabolism, and aging. The precise structure of SIRT1 is vital for function, as this determines substrate specificity, enzymatic efficiency, and interactions with regulatory proteins, making SIRT1 a pivotal target for studies investigating age-related diseases, cancer, and metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, COLO205 cells, using SirT1 Rabbit Polyclonal Antibody (EAB13298) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 82 kDa; Observed band size: 110-130 kDa |
