| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IHC-P, IF/ICC, ELISA | 89 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:10000-50000 IP=1:100-500 IHC-P=1:100-500 IF/ICC=1:200-1000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | KAP1/TIF1β Mouse Monoclonal Antibody detects endogenous levels of KAP1/TIF1β protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
KAP1 also known as TF1β, TRIM28 is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. KAP1 plays a crucial role in transcriptional regulation by interacting with the Kruppel-associated box (KRAB) repression domain found in various transcription factors. This interaction is vital for mediating transcriptional repression, which is essential for processes such as cellular differentiation and response to stress. The human KAP1 gene is located on chromosome 19q13.4 and encodes an 835 amino acid nuclear protein, underscoring its importance in gene expression regulation and cellular homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293 cells, using KAP1/TIF1β Mouse Monoclonal Antibody (EAB21231) at 1:20000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 89 kDa; Observed band size: 100 kDa  Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues, using KAP1/TIF1β Mouse Monoclonal Antibody (EAB21231) at 1:200 dilution.
 Immunofluorescence analysis of HepG2 cells, using KAP1/TIF1β Mouse Monoclonal Antibody (EAB21231) at 1:400 dilution.
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