| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 20 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-MYL9 (Thr18/Ser19) Rabbit Monoclonal Antibody detects endogenous levels of MYL9 only when phosphorylated at Thr18/Ser19. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MYL9 also known as LC20, MLC2, MRLC1 plays a crucial role in muscle contraction regulation by interacting with myosin heavy chains and actin filaments. MYL9 is primarily located in the cytoplasm of smooth muscle and non-muscle cells, where MYL9 is essential for the contractile function. Phosphorylation of MYL9 by myosin light chain kinase (MLCK) in the presence of calcium and calmodulin enhances the actin-activated myosin ATPase activity, which is vital for muscle contraction and cellular motility. Additionally, MYL9 is found in striated skeletal muscle, where MYL9′s precise function is still being elucidated, highlighting MYL9′s importance in both smooth and striated muscle physiology. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with Calyculin A, using Phospho-MYL9 (Thr18/Ser19) Rabbit Monoclonal Antibody (EAB22009) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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