| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 53 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Tpl2 (Thr290) Rabbit Polyclonal Antibody detects endogenous levels of Tpl2 protein only when phosphorylated at Thr290. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Tpl2 also known as Cot is an oncogene that encodes a member of the serine/threonine protein kinase family. Tpl2 plays a crucial role in cell signaling pathways, particularly in activating the mitogen-activated protein kinase (MAPK) pathway, which regulates cellular processes like proliferation, differentiation, and survival. Tpl2 protein is primarily located in the cytoplasm, where Tpl2 interacts with other signaling molecules to modulate cellular responses to external stimuli. Notably, Tpl2 has been implicated in T lymphocyte activation, highlighting Tpl2′s importance in immune responses. Additionally, Tpl2 protein undergoes post-translational modifications that influence activity and interactions, further underscoring Tpl2′s role in cellular signaling networks. Overexpression of Tpl2 induces MAP kinase activation, and Tpl2′s function is intricately linked to proteins such as Ras and Raf-1, suggesting Tpl2 may form a multimeric complex that regulates downstream signaling events. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293 cells treated with UV, using Phospho-Tpl2 (Thr290) Rabbit Polyclonal Antibody (EAB10585) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 53 kDa; Observed band size: 60 kDa |
