www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 80 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IKKε/IKBKE Rabbit Monoclonal Antibody detects endogenous levels of IKKε/IKBKE protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IKKε also known as IKBKE, IKKI is a serine/threonine kinase that plays a crucial role in immune response by regulating the activation of transcription factor NFκB. IKKε is primarily expressed in immune cells and is induced by stimuli such as lipopolysaccharide and proinflammatory cytokines like TNFα, IL-1, and IL-6. IKKε is essential for phosphorylation of IκBα at specific serine residues, which leads to degradation of IκB and subsequent release and activation of NFκB. IKKε′s ability to modulate NFκB signaling is significant, as NFκB is involved in various cellular processes, including inflammation, immune response, and cell survival. Overexpression of IKKε has been linked to enhanced phosphorylation of IκBα and increased NFκB activity, indicating a potential role as an IκB kinase in immune regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji, Mouse spleen cells, using IKKε/IKBKE Rabbit Monoclonal Antibody (EAB21896) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
