| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 44, 42 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Erk1/2 (Tyr222/205) Rabbit Polyclonal Antibody detects endogenous levels of only when phosphorylated at Tyr222/205. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
The ERK proteins, which include ERK 1 and ERK 2, play a crucial role in mediating the activation of signal transduction pathways triggered by growth factors, hormones, and neurotransmitters. These proteins are located in the cytoplasm and translocate to the nucleus upon activation, where they regulate gene expression and influence cell proliferation, differentiation, and survival. The dual phosphorylation of ERK at specific threonine and tyrosine residues is essential for their full enzymatic activation, allowing them to phosphorylate downstream targets on serine and threonine residues. Key upstream regulators of ERK include MAP kinase kinase (MEK), MEK kinase, and Raf-1, which are vital for the proper functioning of the MAPK signaling cascade. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, Mouse lung cells, using Phospho-Erk1/2 (Tyr222/205) Rabbit Polyclonal Antibody (EAB14805) at 1:500 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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