| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P | 78 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PKCγ (Thr514) Rabbit Monoclonal Antibody detects endogenous levels of PKCγ protein only when phosphorylated at Thr514 |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKCγ also known as PKCgamma, PRKCG is a member of the protein kinase C family. PKCγ plays a crucial role in regulating numerous cellular processes such as cell growth, differentiation, and apoptosis. A C1 domain that binds diacylglycerol and phorbol esters characterizes PKCγ structure, allowing activation in response to specific signaling molecules, making PKCγ essential for mediating cellular responses to growth factors and hormones. Distinct expression patterns of PKCγ across different tissues highlight PKCγ importance in tissue-specific signaling pathways, and PKCγ ability to interact with various proteins, including Ras and Rho GTPases, underscores PKCγ role in modulating cytoskeletal dynamics and cell motility. PKCγ versatility in cellular signaling makes PKCγ a significant target for research into cancer and other diseases, where PKCγ dysregulation can lead to altered cell behavior and contribute to tumorigenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using Phospho-PKCγ (Thr514) Rabbit Monoclonal Antibody (EAB22085) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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