| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 35 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Osteopontin/OPN Rabbit Polyclonal Antibody detects endogenous levels of Osteopontin/OPN protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Osteopontin also known as OPN, SPP1, is involved in the attachment of osteoclasts to the mineralized bone matrix. Osteopontin plays a crucial role in various biological processes. Post-translational modifications of OPN, such as phosphorylation and glycosylation, significantly influence molecular weight and functional properties, enabling interactions with specific receptors and proteins to modulate cell adhesion, migration, and immune responses. OPN monoclonal antibody (AKm2A1) helps researchers study this protein, which is predominantly produced by osteoblasts and synthesized in brain and kidneys, demonstrating importance in both skeletal and non-skeletal tissues. |
| Product Image Gallery | |
 Western blot analysis of extracts on LOVO, NIH/3T3 cells, using Osteopontin/OPN Rabbit Polyclonal Antibody (EAB14334) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 35 kDa; Observed band size: 60 kDa |
