| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 265 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cleaved-Notch2 (Asp1733) Rabbit Polyclonal Antibody detects endogenous levels of fragment of activated Notch2 protein resulting from cleavage adjacent to Asp1733. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Notch2 is a member of the Notch family. Notch 2 is a crucial component of the Notch signaling pathway, which is integral to cell communication and plays a significant role in regulating cell fate decisions during development. Notch 2 is primarily located in the cell membrane, where this receptor interacts with ligands such as Jagged and Delta, facilitating essential processes like cell differentiation and tissue development. Proper functioning of Notch 2 is vital, as dysregulation can lead to various developmental disorders and has been implicated in cancer progression. Notch 2′s interaction with ligands not only influences cell fate but also contributes to stem cell population maintenance and immune response regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293T cells, using Cleaved-Notch2 (Asp1733) Rabbit Polyclonal Antibody (EAB14161) at 1:500 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 265 kDa; Observed band size: 110 kDa |
