| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 149 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NEDD4 Rabbit Monoclonal Antibody detects endogenous levels of NEDD4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NEDD4 is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. NEDD4 plays a significant role in maintaining Golgi structure and function, essential for proper protein sorting and trafficking within cells. NEDD4 interacts with WW domains of several HECT-type E3 ubiquitin ligases, suggesting involvement in ubiquitination processes that regulate protein degradation and cellular signaling pathways. NEDD4 shows high expression in neurons surrounding injury sites, indicating potential neuroprotective functions through ubiquitination modulation, which may contribute to neuronal survival strategies. NEDD4 expression and interaction with E3 ubiquitin ligase Itch increases upon T cell activation, highlighting relevance in immune responses. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, HCT116, C6 cells, using NEDD4 Rabbit Monoclonal Antibody (EAB22584) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 149 kDa; Observed band size: 130 kDa |
