| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 74 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Lamin A/C Rabbit Polyclonal Antibody detects endogenous levels of Lamin A/C protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Lamin A/C plays a crucial role in maintaining the structural integrity of the nuclear envelope, which is essential for proper cellular function and organization. Lamin A/C is located in the nuclear lamina, a dense fibrillar network inside the nucleus, where Lamin A/C provides mechanical support and regulates important processes such as DNA replication and cell division. The significance of Lamin A/C extends beyond structural support; Lamin A/C is also involved in chromatin organization and gene expression regulation. Disruptions in Lamin A/C function can lead to various diseases, including muscular dystrophies and progeria, highlighting Lamin A/C′s importance in cellular health and longevity. Lamin A is cleaved by the protease Mch2 during apoptosis, while Lamin C, a splice variant of Lamin A, shares a high degree of similarity with Lamin A but differs at the carboxy-terminus, which may influence specific interactions and functions within the cell. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc, NIH/3T3 cells, using Lamin A/C Rabbit Polyclonal Antibody (EAB10349) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 74 kDa; Observed band size: 74,65 kDa  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Lamin A/C Rabbit Polyclonal Antibody (EAB10349) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using Lamin A/C Rabbit Polyclonal Antibody (EAB10349) at 1:200 dilution.
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