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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 137 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IRS-2 Rabbit Polyclonal Antibody detects endogenous levels of IRS-2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IRS-2(Insulin Receptor Substrate 2) plays a crucial role as a signaling intermediate downstream of several receptors, including insulin, insulin-like growth factor 1, interleukin-4, interleukin-9, and interleukin-13.iRS-2 is involved in activating mitogen-activated protein kinase (MAPK) and protein kinase B (PKB) pathways, which are essential for cellular responses to growth factors. In IRS-2-deficient mice, a significant reduction in total phosphoinositide 3-kinase activity and subsequent loss of protein kinase C (PKC) zeta activation can lead to type 2 diabetes, highlighting IRS-2′s importance in metabolic regulation. IRS-2 translocation to nuclei of mouse embryo fibroblasts expressing insulin-like growth factor 1 receptor underscores its multifaceted role in cellular signaling and gene expression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2 cells, using IRS-2 Rabbit Polyclonal Antibody (EAB14229) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 137 kDa; Observed band size: 170 kDa |
