| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 11 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IL-8 Rabbit Polyclonal Antibody detects endogenous levels of IL-8 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IL-8(Interleukin-8) also known as CXCL8, IL8 is a crucial member of the C-X-C family of chemokines, primarily functioning as a potent chemoattractant for neutrophils, which are essential for the immune response. IL-8 is secreted by various cell types, including macrophages and epithelial cells, and plays a significant role in mediating inflammation and the immune response to infection. Notably, IL-8 is produced as a 99 amino acid precursor that is cleaved to yield a 72 amino acid, biologically active form that is nonglycosylated. The dynamic equilibrium between IL-8 monomers and dimers, both in solution and bound to cell surfaces, is critical for regulating chemotaxis and receptor signaling. Research indicates that IL-8 dimerization acts as a negative regulator of IL-8 receptor function, which is vital for maintaining the balance of immune responses. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, PC12, HEK293 cells, using IL-8 Rabbit Polyclonal Antibody (EAB14194) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using IL-8 Rabbit Polyclonal Antibody (EAB14194) at 1:100 dilution.
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