| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 16 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IBA1 Rabbit Polyclonal Antibody detects endogenous levels of IBA1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IBA1 also known as AIF1 is a protein that binds actin and calcium. IBA1 is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. IBA1 plays a significant role in macrophage activation and function, making Iba1 essential for immune responses and tissue repair. IBA1 contains two EF-hand domains, which are vital for calcium-binding capability, and is induced by various cytokines and interferons, highlighting IBA1′s importance in inflammatory processes. In unstimulated state, IBA1 colocalizes with actin, but upon activation, Iba1 translocates to lamellipodia, facilitating cellular movement and response to injury. Additionally, IBA1 serves as a specific marker for human microglia and is expressed by macrophages in injured skeletal muscle, underscoring Iba1′s relevance in neuroinflammation and muscle regeneration. |
| Product Image Gallery | |
 Western blot analysis of extracts on U2OS cells, using IBA1 Rabbit Polyclonal Antibody (EAB14164) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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