| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 52 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HNF4α Rabbit Polyclonal Antibody detects endogenous levels of HNF4α protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HNF4α also known as HNF4alpha, HNF4A is a nuclear transcription factor which binds DNA as a homodimer. HNF4α controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. HNF4α is predominantly expressed in the liver, kidney, pancreas, intestine, testis, and colon, influencing the transcription of numerous hepatocyte-specific genes such as cytochrome P450 enzymes and alpha-1 antitrypsin. Mutations in HNF4α are associated with maturity-onset diabetes of the young type 1 (MODY1), underscoring HNF4α′s importance in maintaining glucose homeostasis. HNF4α interacts with other hepatocyte nuclear factors like HNF1 and HNF3 to regulate gene expression during early embryonic development and organogenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HT29 cells, using HNF4α Rabbit Polyclonal Antibody (EAB14014) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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