| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 41 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HAO1 Rabbit Polyclonal Antibody detects endogenous levels of HAO1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HAO1 also known as GOX1 is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. HAO1 plays a significant role in organic acid metabolism through 2-hydroxyacid oxidase activity, which requires a flavin cofactor to catalyze the oxidation of 2-hydroxyacids to 2-ketoacids while simultaneously reducing oxygen to hydrogen peroxide. Notably, HAO1 preferentially oxidizes glycolate, producing glyoxylate, which is vital for the peroxisomal synthesis of glycine. Additionally, HAO1 can convert glyoxylate to oxalate, linking HAO1 to the pathophysiology of hyperoxaluria type 1, a condition characterized by the accumulation of oxalate due to defects in the peroxisomal enzyme AGXT. This accumulation can lead to the formation of calcium oxalate precipitates in the kidneys, posing serious health risks. HAO1′s ability to interact with various substrates, including 2-hydroxy fatty acids and L-α-hydroxy acids, further underscores HAO1′s importance in metabolic pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse liver, Rat liver cells, using HAO1 Rabbit Polyclonal Antibody (EAB14143) at 1:500 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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