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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 27 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Granzyme B Rabbit Polyclonal Antibody detects endogenous levels of Granzyme B protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Granzyme B also known as GZMB is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Granzyme B plays a crucial role in the immune response, particularly in the induction of apoptosis in target cells, which is essential for maintaining immune homeostasis and eliminating infected or malignant cells. This serine protease is primarily located within cytolytic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, where granzyme B is stored as an inactive proenzyme until release during degranulation. Upon activation, granzyme B cleaves and activates caspases, such as caspase-3, caspase-6, caspase-7, and caspase-9, leading to a cascade of events that culminate in programmed cell death. The precise localization of granzyme B within cytolytic granules ensures direct delivery to target cells during immune responses, enhancing the efficiency of apoptosis induction |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse lung, Mouse spleen cells, using Granzyme B Rabbit Polyclonal Antibody (EAB14180) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 27 kDa; Observed band size: 38 kDa  Immunohistochemical analysis of paraffin-embedded Human spleen tissue, using Granzyme B Rabbit Polyclonal Antibody (EAB14180) at 1:200 dilution.
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